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原文連結
論文資訊
- 類型：已發表論文
- 日期：2007
摘要
Small regulatory microRNAs (miRNAs) are encoded in long precursors and are released from them during processing by cleavage within partially duplexed stem–loop structures. In the present work we investigated the role of the Arabidopsis nuclear RNA-binding 蛋白質 HYL1 and the nuclear RNase III enzyme DCL1 in processing of primary miRNA (pri-miR171a). The miR171a gene is complex, with multiple transcription start sites, as well as alternative splicing of exons and alternative polyadenylation sites. Both HYL1 and DCL1 蛋白質s are required for processing of the major pri-miR171a, spliced and polyadenylated forms of which accumulate in plants homozygous for 突變s in either gene, but not in wild-type plants. In transiently transfected Arabidopsis protoplasts, HYL1-mCherry and YFP-DCL1 fusion 蛋白質s coloca
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