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原文連結
論文資訊
- 類型：已發表論文
- 日期：2008
摘要
We have used fluorescence resonance energy transfer and co-immunoprecipitation to analyze the interactions among the alpha, beta, and gamma 1 subunits of the Arabidopsis heterotrimeric G 蛋白質. Using cyan and yellow fluorescent 蛋白質 fusion constructs, we show that overexpressed G(gamma 1) localizes to protoplast membranes, but G beta exhibits membrane localization only when the G gamma(1) 蛋白質 is co-overexpressed. Overexpressed G alpha shows membrane localization unaccompanied by overexpression of either G beta or G gamma(1). We detect fluorescence resonance energy transfer between G beta and G gamma(1) in the absence of G alpha overexpression and between G alpha and G gamma(1) but only when all three subunits are co-overexpressed. Both G alpha and G beta are associated with large macromolecul
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