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原文連結
論文資訊
- 類型：已發表論文
- 日期：2010
摘要
RNA silencing is a eukaryotic mechanism involved in several cellular processes, one example being a sequence-specific antiviral defense. Many plant 病毒es have developed counterdefensive 蛋白質s that in many instances are multifunctional, such as helper component protease (HC-Pro) of Tobacco etch 病毒 (TEV). In a previous work, a collection of mutants with amino acid replacements in TEV HC-Pro was generated, and their effects in the capacity of suppressing RNA silencing were quantified in a transient expression assay. In this study, three 突變s that caused a reduction in suppression activity and three that increased it were used to create replicate experimental lineages that were evolved through serial passages. We have evaluated the number of genotypic changes that occurred during 演化 in HC-Pro and
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