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原文連結
論文資訊
- 類型：已發表論文
- 日期：2011
摘要
A biotechnological application of artificial microRNAs (amiRs) is the generation of plants that are resistant to 病毒 infection. This resistance has proven to be highly effective and sequence specific. However, before these transgenic plants can be deployed in the field, it is important to evaluate the likelihood of the 湧現 of resistance-breaking mutants. Two issues are of particular interest: (i) whether such mutants can arise in nontransgenic plants that may act as reservoirs and (ii) whether a suboptimal expression level of the transgene, resulting in subinhibitory concentrations of the amiR, would favor the 湧現 of escape mutants. To address the first issue, we experimentally evolved independent lineages of Turnip mosaic 病毒 (TuMV) (family Potyviridae) in fully susceptible wild-type Arabidop
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