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原文連結
論文資訊
- 類型：已發表論文
- 日期：2012
摘要
Measuring fitness with precision is a key issue in 演化ary biology, particularly in studying 突變s of small effects. It is usually thought that sampling error and drift prevent precise measurement of very small fitness effects. We circumvented these limits by using a new combined approach to measuring and analyzing fitness. We estimated the 突變al fitness effect (MFE) of three independent mini-Tn10 transposon insertion 突變s by conducting competition experiments in large 族群s of Escherichia coli under controlled laboratory conditions. Using flow cytometry to assess genotype frequencies from very large samples alleviated the problem of sampling error, while the effect of drift was controlled by using large 族群s and massive replication of fitness measures. Furthermore, with a set of four competition e
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