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原文連結
論文資訊
- 類型：已發表論文
- 日期：2013
摘要
Defective interfering (DI) 病毒es are thought to cause oscillations in 病毒 levels, known as the 'Von Magnus effect'. Interference by DI 病毒es has been proposed to underlie these dynamics, although experimental tests of this idea have not been forthcoming. For the baculo病毒es, insect 病毒es commonly used for the expression of heterologous 蛋白質s in insect cells, the molecular mechanisms underlying DI generation have been investigated. However, the dynamics of baculo病毒 族群s harboring DIs have not been studied in detail. In order to address this issue, we used quantitative real-time PCR to determine the levels of helper and DI 病毒es during 50 serial passages of Autographa californica multiple nucleopolyhedro病毒 (AcMNPV) in Sf21 cells. Unexpectedly, the helper and DI 病毒es changed levels largely in phase,
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