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原文連結
論文資訊
- 類型：已發表論文
- 日期：2014-02-12
摘要
Eulimnogammarus verrucosus is an amphipod endemic to the unique ecosystem of Lake Baikal and serves as an emerging model in ecotoxicological studies. We report here on a survey sequencing of its 基因組 as a first step to establish sequence resources for this 物種. >From a single lane of paired-end sequencing data, we estimated the 基因組 size as nearly 10 Gb and we obtained an overview of the repeat content. At least two-thirds of the 基因組 are non-unique DNA, and a third of the genomic DNA is composed of just five families of repetitive elements, including low-complexity sequences. Attempts to use off-the-shelf assembly tools failed on the available low-coverage data both before and after removal of highly repetitive components. Using a seed-based approach we nevertheless assembled short contigs co
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