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原文連結
論文資訊
- 類型：已發表論文
- 日期：2014-03-27
摘要
Background: Differential RNA sequencing (dRNA-seq) is a high-throughput screening technique designed to examine the architecture of 細菌l operons in general and the precise position of transcription start sites (TSS) in particular. Hitherto, dRNA-seq data were analyzed by visualizing the sequencing reads mapped to the reference 基因組 and manually annotating reliable positions. This is very labor intensive and, due to the subjectivity, biased. Results: Here, we present TSSAR, a tool for automated de novo TSS annotation from dRNA-seq data that respects the statistics of dRNA-seq libraries. TSSAR uses the premise that the number of sequencing reads starting at a certain genomic position within a transcriptional active region follows a Poisson distribution with a parameter that depends on the loca
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