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原文連結
論文資訊
- 類型：已發表論文
- 日期：2015-12-01
摘要
Background: Understanding the causes and consequences of phenotypic variability is a central topic of 演化ary biology. 突變s within non-coding cis-regulatory regions are thought to be of major effect since they affect the expression of downstream genes. To address the 演化ary potential of 突變s affecting such regions in RNA 病毒es, we explored the fitness properties of 突變s affecting the 5'-untranslated region (UTR) of a prototypical member of the picorna-like superfamily, Tobacco etch 病毒 (TEV). This 5' UTR acts as an internal ribosomal entry site (IRES) and is essential for expression of all viral genes. Results: We determined in vitro the folding of 5' UTR using the selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) technique. Then, we created a collection of single-nucleotide sub
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