摘要 In the experimental 演化 of microbes such as Escher

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論文資訊

• 類型：已發表論文
• 日期：2016

摘要

In the experimental 演化 of microbes such as Escherichia coli, many replicate 族群s are evolved from a common ancestor. Freezing a 族群 sample supplemented with the cryoprotectant glycerol permits later analysis or restarting of an 演化 experiment. Typically, each evolving 族群, and thus each sample archived in this way, consists of many unique genotypes and phenotypes. The effect of archiving on such a heterogeneous 族群 is unknown. Here, we identified optimal archiving conditions for E. coli. We also used 基因組 sequencing of archived samples to study the effects that archiving has on genomic 族群 多樣性. We observed no allele substitutions and mostly small changes in allele frequency. Nevertheless, principal component analysis of 基因組-scale allelic 多樣性 shows that archiving affects 多樣性 across many loci. We s

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