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原文連結
論文資訊
- 類型：已發表論文
- 日期：2021-07-12
摘要
Translational errors during 蛋白質 synthesis cause phenotypic 突變s that are several orders of magnitude more frequent than DNA 突變s. Such phenotypic 突變s may affect adaptive 演化 through their interactions with DNA 突變s. To study how mistranslation may affect the adaptive 演化 of evolving 蛋白質s, we evolved 族群s of green fluorescent 蛋白質 (GFP) in either high-mistranslation or low-mistranslation Escherichia coli hosts. In both hosts, we first evolved GFP under purifying selection for the ancestral phenotype green fluorescence, and then under directional selection toward the new phenotype yellow fluorescence. High-mistranslation 族群s evolved modestly higher yellow fluorescence during each generation of 演化 than low-mistranslation 族群s. We demonstrate by high-throughput sequencing that elevated mistranslation
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