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論文資訊
- 類型：已發表論文
- 日期：2022-02-01
摘要
Cell-free expression (CFE) systems are one of the main platforms for building synthetic cells. A major drawback is the orthogonality of cell-free systems across 物種. To generate a CFE system compatible with recently established minimal cell constructs, we attempted to optimize a Mycoplasma bacterium-based CFE system using lysates of the 基因組-minimized cell JCVI-syn3A (Syn3A) and its close 系統發育 relative Mycoplasma capricolum (Mcap). To produce mycoplasma-derived crude lysates, we systematically tested methods commonly used for 細菌, based on the S30 protocol of Escherichia coli. Unexpectedly, after numerous attempts to optimize lysate pro- duction methods or composition of feeding buffer, none of the Mcap or Syn3A lysates supported cell-free gene expression. Only modest levels of in vitro trans
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