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論文資訊
- 類型：已發表論文
- 日期：2022-07-07
摘要
蛋白質 abundance affects the 演化 of 蛋白質 genotypes, but we do not know how it affects the 演化 of 蛋白質 phenotypes. Here we investigate the role of 蛋白質 abundance in the evolvability of green fluorescent 蛋白質 (GFP) towards the novel phenotype of cyan fluorescence. We evolve GFP in E. coli through multiple cycles of 突變 and selection and show that low GFP expression facilitates the 演化 of cyan fluorescence. A 計算 model whose predictions we test experimentally helps explain why: lowly expressed 蛋白質s are under stronger selection for proper folding, which facilitates their evolvability on short 演化ary time scales. The reason is that high fluorescence can be achieved by either few 蛋白質s that fold well or by many 蛋白質s that fold less well. In other words, we observe a synergy between a 蛋白質’s scarcity and its sta
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