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原文連結
論文資訊
- 類型：已發表論文
- 日期：2022-10-13
摘要
The spectrophotometer has been used for decades to measure the density of 細菌l 族群s as the turbidity expressed as optical density-OD. However, the OD alone is an unreliable metric and is only proportionately accurate to cell titers to about an OD of 0.1. The relationship between OD and cell titer depends on the configuration of the spectrophotometer, the length of the light path through the culture, the size of the 細菌l cells, and the cell culture density. We demonstrate the importance of plate reader calibration to identify the exact relationship between OD and cells/mL. We use four 細菌l genera and two sizes of micro-titer plates (96-well and 384-well) to show that the cell/ml per unit OD depends heavily on the 細菌l cell size and plate size. We applied our calibration curve to real growth curv
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