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原文連結
論文資訊
- 類型：已發表論文
- 日期：2023-01-11
摘要
病毒es are obligate intracellular parasites that have co-evolved with their hosts to establish an intricate 網絡 of 蛋白質–蛋白質 interactions. Here, we followed a high-throughput yeast two-hybrid screening to identify 378 novel 蛋白質–蛋白質 interactions between turnip mosaic 病毒 (TuMV) and its natural host Arabidopsis thaliana. We identified the RNA-dependent RNA polymerase NIb as the viral 蛋白質 with the largest number of contacts, including key salicylic acid-dependent transcription regulators. We verified a subset of 25 interactions in planta by bimolecular fluorescence complementation assays. We then constructed and analyzed a 網絡 comprising 399 TuMV-A. thaliana interactions together with intra病毒 and intrahost connections. In particular, we found that the host 蛋白質s targeted by TuMV are enriched in diffe
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